Prof. RNDr. Vladimír Sklenář, DrSc.

Education, Scientific and Pedagogical Carrier

Faculty of Science, Masaryk University, Brno, Chemistry, 1974.
RNDr degree: Department of Theoretical and Physical Chemistry
Faculty of Science, Masaryk University, Brno, 1975.
CSc. degree (equivalent to PhD.), Technical University, Brno, 1984.
Associate professor, Faculty of Science, Masaryk University, Brno, 1992
DrSc. degree, Institute of Chemical Technology, Prague, 1996
Full professor, Faculty of Science, Masaryk University, Brno, 1997

Employment and Scientific Sojourns

Institute of Scientific Instruments,
Department of radiofrequency spectroscopy, Kralovopolska 147, 612 64 Brno. associate scientist, scientist, principal research scientist 1976-1995
Laboratory of Chemical Physics, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health,
Rockville Pike, Bethesda, MD 20892, USA, visiting fellow 1986-87
University of California, Los Angeles, Department of Chemistry and Biochemistry,
Hilgard Av., Los Angeles, CA 90024-1569, USA from r.1989 continuous cooperation, visiting scholar 1989-95
Marion Merrell Dow Research Institute, Biophysics Core Facility,
16, rue d'Ankara, 67080 Strasbourg, France,visiting scientist,1992-95

Professional Interests

NMR methods, development of multi-dimensional FT NMR techniques, spin system dynamics, theoretical description of spin system behaviour, gradient-enhanced NMR spectroscopy, multiple-resonance techniques, Ab Initio calculations of NMR parameters, NMR-based structural studies of nucleic acids and proteins.

LIST OF RECENT PAPERS - (long list)

Sklenář, V., Masse, J.E., and Feigon, J.; J. Magn. Reson. 1999, 137, 345-349: HCCCH Experiments for Through-Bond Correlation of Thymine Resonances in C-13 Labeled DNA Oligonucleotides.

Hud, N.V., Schultze, P., Sklenář, V., and Feigon, J., J. Mol. Biol. 1999, 285, 233-243: Binding Sites and Dynamics of Ammonium Ions in a Telomere Repeat DNA Quadruplex.

Marek, R., Humpa, O., Dostál, J., Slavík, J., and Sklenář, V., Magn. Reson. Chem. 1999 37, 195-202: ¹⁵N NMR Study of Isoquinoline Alkaloids.

Hud, N.V., Sklenář, V. and Feigon, J., J. Mol. Biol. 1999, 286, 651-660: Localization of Ammonium Ions in the Minor Groove of DNA Duplexes in Solution and the Origin of DNA A-Tract Bending.

Czernek J. and Sklenář V., J. Phys. Chem. A 1999, 103, 4089-4093: Ab Initio Calculations of ¹H and ¹³C Chemical Shifts in Anhydrodeoxythymidines.

Marek, R., Toušek, J., Dostál, J., Slavík, J., Dommisse, R., and Sklenář, V.; Magn. Reson. Chem., 1999, 37, 781-787: ¹H and ¹³C NMR Study of Quaternary Benzo[c]phenanthridine Alkaloids.

Czernek J. and Sklenář, V., Chem. Listy 2000, 94, 90-96: Ab initio výpočty chemických posunů v biomolekulách.

Fiala, R., Czernek, J. and Sklenář, V.: J. Biomol . NMR 2000, 16, 291-302: Transverse Relaxation Optimized Triple-Resonance NMR Experiments for Nucleic Acids.

Damborský, J., Damborská, M., Štípek, S., Jesenská, A., Trantírek, L., and Sklenář, V., Folia Microbiologica 2000, 45, 35-40: Effect of the Carbon Source on Assesment of Degrading Bacteria with the Spread-Plating Technique during in situ Bioremediation.

Czernek, J., Lang, J., and Sklenář, V., J. Phys. Chem. A 2000, 104, 2788-2792: Ab Initio Calculations of Spin-Spin Coupling Constants Shifts in Anhydrodeoxythymidines.

Czernek, J., Fiala, R., and Sklenář, V., J. Magn. Reson. 2000, 145, 142-146: Hydrogen Bonding Effects on the ¹⁵N and ¹H Shielding Tensors in Nucleic Acids Base Pairs.

Trantírek, L., Štefl, R., Vorlíčková, M., Koča, J., Sklenář, V., and Kypr, J., J. Mol. Biol. 2000, 297, 907-922: An A-type Double Helix of DNA Having B-type Puckering of the Deoxyribose Rings.

Trantírek, L., Urbášek, M., Štefl, R., Feigon, J. and Sklenář, V., J. Amer. Chem. Soc. 2000, 122, 10454-10455: A Method for Direct Determination of Helical Parameters in Nucleic Acids Using Residual Dipolar Couplings

Trantírek, L., Hynková, K., Nagata, Y., Murzin, A., Ansorgova, A., Sklenář, V. and Damborský, J., J. Biol. Chem. 2001,267, 7734-7740: Reaction Mechanism and Stereochemistry of J-hexachlorocyclohexane Dehydrochlorinase LinA.

Štefl, R.,Trantírek, L., Vorlíčková,M., Koča, J., Sklenář,V., and Kypr, J., J. Mol. Biol. 2001, 307, 513-524: A-like Guanine-Guanine Stacking in the Aqeuous DNA Duples of d(GGGGCCCC).

Žídek, L., Štefl, R., Sklenář, V., Current Opinion in Structural Biology 2001, 11, 275-281:NMR methodology for the study of nucleic acids.

Žídek, L., Wu, H., Feigon, J., Sklenář, V.: J. Biomol. NMR 2001, 21, 2, 153-160: Measurement of small scalar and resiudal dipolar couplings in purine and pyrimidine bases.

Trantírek, L., Štefl, R., Masse, J., Feigon, J., Sklenář, V.: J. Biomol. NMR 2002, 23, 1, 1-12: Determination of the glycosidic torsion angels in uniformly C-13 labeled nucleic acids from vicinal coupling constants ³J_C2/4-H1' and ³J_C6/8-H1'.

Fiala, R. and Sklenář, V., Ed. O. Zerbe in Series Methods and Principles in Medicinal Chemistry, Eds. Mannhold, R., Kubinyi, H., Folkers, G.: NMR od Nucleic Acids 2002, 121-145.

Padrta, P., Štefl, R., Králík, L., Žídek, L., Sklenář, V.: J. Biomol. NMR 2002, 24, 1, 1-14: Refinement of d(GCGAAGC) Hairpin Structure Using One- and Two-Bond Residual Dipolar Couplings.

Sychrovský, V., Vacek, J., Hobza, P., Žídek, L., Sklenář, V., Cremer, D.: J. Phys. Chem. B 2002, 106, 29, s. 10242-10250: Exploring the Structure of a DNA Hairpin with the Help of NMR Spin-spin Coupling Constants: An Experimental and Quantum Chemical Investigation.

Veselá, D., Marek R., Ubik, K., Lunerová, K., Sklenář, V., and Suchý, V.: Phytochemistry 2002, 61, 967-970: Dracophane, a Novel Metacyclophane Derivative from Resin of Dracaena Cinnabari Balf.

Munzarová, M., Sklenář, V.: J. Amer. Chem. Soc. 2002, 124, 10666-10667: Three-Bond Sugar-Base Coupling in Purine versus Pyrimidine Nucleosides: A DFT Study of Karplus Relationships for ³J_C2/4-H1' and ³J_C6/8-H1' in DNA.

Padrta, P. and Sklenář, V.: J. Biol. NMR 2002, 24, 4, 339-349: Program MULDER - A Tool for extracting torsion angles from NMR data.

Sychrovský, V., Schneider, B., Hobza, P., Žídek, L., Sklenář, V.: Phys. Chem.Chem. Phys. 2003, 5, 734-739: The Effect of Water on NMR Spin-Spin Couplings in DNA: Improvement of Calculated Values by Application of Two Solvent Models.

Munzarová, M., Sklenář, V.: J. Amer. Chem. Soc. 2003, 125, 3649-3658: A DFT Analysis of NMR Scalar Interactions Across the Glycosidic Bond in DNA.

Marek, R., Sečkářová, P., Hulová, D., Marek, J., Dostál, J., Sklenář, V.: J. Nat. Prod. 2003, 66, 4, 481-486: Palmatine and Berberine Isolation Artifacts..

Žídek, L., Padrta, P., Chmelík, J. Jr., and Sklenář, V.:J. Magn. Reson. 2003, 162, 385-395: Internal Consistency of NMR Data Obtained in Partially Aligned Biomacromolecules.

Vorlíčková, M., Kypr, J., and Sklenář, V.: Encyclopedia of Analytical Science 2^nd Edition, Academic Press: Nucleic Acids: (c) spectroscopic methods.

Křížová, H., Žídek, L., Stone, M., Novotny, M.V., and Sklenář, V., J. Biomol. NMR 2004, 28, 4, 369-384: Temperature Dependent Backbone Dynamics of Major Urinary Protein-I Complexed with the Pheromone 2-sec-Butyl-4,5-dihydrothiazole.

Štefl, R., Wu, H., Ravindranathan, S., Sklenář, V., and Feigon, J.:Proc. Nat. Acad. Sci. 2004, 101, 5, 1177-1182: DNA A-tract bending in three dimensions-solving the dA4T4 versus dT4A4 conundrum.

Fiala, R., Munzarová, M., and Sklenář, V.:J. Biomol. NMR 2004, 29, 477-490. Experiments for Correlating Quaternary Carbons in RNA Bases.

Sečkářová, P., Marek, R., Maliňáková, K., Kohlemainen, E., Hocková, , D., Hocek, M., and Sklenář, V.: Tetrahedron Lett. 2004, 45, 6259-6263. Direct determination of tautomerism in purine derivatives by low-temerature NMR spectroscopy.

Marek, R. and Sklenář, V.: Ann. Rep. NMR Spect. 2005, 54, 201-242: NMR Studies of Purines.

National Centre for Biomolecular Research
NMR Laboratory
Faculty of Science, Masaryk University
Kotlarska 2, CZ-611 37 Brno
tel.:+420 - 549 497 022
fax :+420 - 549 492 556
e-mail: sklenar@chemi.muni.cz

last modification: Tue Mar 8 20:43:32 MET 2005